Azido-PEG4-NHS Ester疊氮基-PEG4-NHS 酯是一種標(biāo)記試劑，可用于通過(guò)穩(wěn)定的酰胺鍵修飾具有疊氮基團(tuán)的伯胺基團(tuán)（例如賴氨酸殘基的側(cè)鏈或氨基硅烷包被的表面）。疊氮化物 （N3） 基團(tuán)通過(guò) CuAAC 與末端炔烴反應(yīng)反應(yīng)，通過(guò)無(wú)銅點(diǎn)擊反應(yīng)與應(yīng)變環(huán)辛炔（例如 DBCO 或 BCN 化合物）反應(yīng)，或通過(guò)稱為 Staudinger 化學(xué)的機(jī)制與磷化氫標(biāo)記分子反應(yīng)，從而實(shí)現(xiàn)生物樣品中衍生化分子的高效和特異性偶聯(lián)。

Azido-PEG4-NHS Ester is a labeling reagent that can be used to modify primary amine groups (e.g., a side chain of lysine residues or aminosilane-coated surfaces) with an azido group via a stable amide bond. The azide (N3) group reacts with terminal alkynes via CuAAC, strained cyclooctynes (e.g. DBCO or BCN compounds) via Cu-free click reaction or with phosphine-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples.